Composite

Part:BBa_K2328040:Design

Designed by: Zhongyi Jiang   Group: iGEM17_TJU_China   (2017-10-14)


HU + smURFP III + Histag.a + RBS III + HO-1 III + pMB1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 840
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 840
    Illegal NgoMIV site found at 330
    Illegal NgoMIV site found at 407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2256


Design Notes

It is a co-expression construction used in B. longum. But since B.longum is a kind of obligate anaerobe, this part is just a test of our project (whether B.longum NC2700 is oxygen resistence or not).


Source

AAAA

References